MB041-EN-1: dNTP Mix, 10 mM each

Chemically synthesized and >99% purity confirmed by HPLC;
Free of human and E. coli DNA;
Free of DNases and RNases;
Free from PCR, qPCR, and cDNA synthesis inhibitors;
Stable for 2 years at –20°C.

MB066-EN-1: dNTP mix, 25 mM each

MB066-EN-2: dNTP set, 100 mM each

Chemically synthesized and >99% purity confirmed by HPLC;
Free of human and E. coli DNA;
Free of DNases and RNases;
Free from PCR, qPCR, and cDNA synthesis inhibitors;
Stable for 2 years at –20°C.
 

MB119-B: 2x hot-start high-fidelity PCR Master Mix, with blue dye
* Simple operation: The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors. The tracking dye and precipitant have been added into the master mix so that the PCR product can be directly loaded for electrophoresis.
* Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches.
* Fast amplification: normally 4-5 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional PCR master mix.
* Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
* Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.
* Features of the hot-start high fidelity DNA polymerase (catalog No. MB118) contained in the 2x hot-start high-fidelity PCR master mix.

MB121: Bst 2G DNA Polymerase, Large Fragment

* Rapid amplification of diverse templates.
* Higher amplification yield.
* Increased tolerance of inhibitors including salts.
* Improved thermostability of the enzyme.
* The cheapest Bst 2.0 DNA Polymerase, Large Fragment in the market.
 

MB122: Bst DNA Polymerase, Large Fragment

* Warm-start reaction system: The reaction process is completed by simply mixing all components at 60 - 65 ℃ for 30 - 60 minutes.
* Multiple options of reading results: The amplification products can be checked with gel electrophoresis, colorimetric methods, and fluorescence intensity methods.
* Potent amplification ability: The upgraded polymerase features strong 5´→3´ DNA polymerase activity and strong strand displacement activity.
* Perfect for assembling RT-LAMP kits: Compatible with various reverse transcriptases from Syd Labs and other commercial suppliers.

MB128: Dye-Based One-Step qRT-PCR Kit

* Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination.
* High detection sensitivity of one step qRT-PCR SYBR green kit: 1 pg total RNA.
* More stable amplification curve with low concentration template.
* Reduced formation of primer dimers and improved specificity of qPCR products.
* Convenient, easy, and quick operation.

MB040-HY2: 2x HY PCR Master Mix, with blue dye

* The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
* The high yield PCR Master Mix has increased amplification robustness, fidelity, yield, and fragment length, as well as the ability to handle difficult or “dirty” templates.
* The tracking dye and precipitant have been added into the PCR PreMix so that the PCR product can be directly loaded for electrophoresis.

Features of the blend of Taq enzyme and a proofreading enzyme contained in the high yield PCR master mix:
* Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
* Proofreading with 5′ exonuclease activity.

MB016-HYT: High Yield Taq DNA Polymerase

* Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results. No visible activity change after storage of the high yield Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
* Proofreading with 5′ exonuclease activity.

MB118: Hot-Start High-Fidelity DNA Polymerase

* Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
* Fast amplification speed: normally ~30 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional Taq DNA polymerase.
* High specificity: Its amplification mismatch rate is 1/53 that of ordinary Taq polymerase and 1/6 that of Pfu polymerase.
* Reproducible results: even with the GC-rich fragments and in the presence of PCR inhibitors.
* Blunt-ended amplified products.
* Low cost: $0.8 per unit. The cheapest hot start high fidelity Taq enzyme in the market.

MB087: Hot Start Taq DNA Polymerase

* High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 5 kb.
* High specificity.
* Reproducible results.
* Terminal transferase activity of adding a single nucleotide (adenosine) at 3' end of the extension product, facilitating TA cloning of PCR products.
* Low cost: $0.19+ per unit. The cheapest hot start Taq enzyme in the market.

MB117: Hot Start Taq DNA Polymerase for Probe-based qPCR

* High amplification specificity and sensitivity for detection of low-copy genes paired with the most suitable buffer optimized for qPCR and PCR.
* For probe-based qPCR, excellent amplification curve within a wide quantitative range, and accurately quantify and detect target genes.
* Compatibility in terms of template type, template GC content, and primer Tm values.
* Good tolerance to impurities and is suitable for use in a variety of testing scenarios.
* Low cost: $0.19+ per unit. The cheapest hot start Taq enzyme in the market.

BP001310-C152: Recombinant E.coli Mismatch-specific uracil DNA-glycosylase (MUG) Enzyme

Source: E. coli-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.